Part:BBa_K4806217:Design

CYPCamC gene for expression in the chloroplast for Chlamydomonas reinhardtii (Phytobrick)

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1205
  Illegal PstI site found at 1477
  Illegal PstI site found at 2201
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 1205
  Illegal PstI site found at 1477
  Illegal PstI site found at 2201
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1205
  Illegal PstI site found at 1477
  Illegal PstI site found at 2201
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1205
  Illegal PstI site found at 1477
  Illegal PstI site found at 2201
  Illegal NgoMIV site found at 3222
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part has been codon optimized for Chlamydomonas reinhardtii and it is compatible with Phytobricks/MoClo parts.

Source

This composite part is a combination of the following parts:

• 1. PSAD-promotor (A1-B1) (BBa_K4806010)
• 2. CTPPSAD-transit peptide (B2) (BBa_K4806014)
• 3. CYPCamC gene (B3-B4) (BBa_K4806002)
• 4. HA-tag (B5) (BBa_K3002017)^*
• 5. tRPL23-terminator (B6-C1) (BBa_K3002006)^*

Additionally, connectors were used to create the MoClo overhangs:

• 1. Connector B1-B2 (BBa_K3002302)^*
• 1. Connector B2-B3 (BBa_K3002303)^*
• 2. Connector B4-B5 (BBa_K3002304)^*
• 3. Connector B5-B6 (BBa_K3002305)^*

The ^* marked parts were not created by us. Our results can be found on the experience page of each part.